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Source: www.ncbi.nlm.nih.gov --- 2 days ago
Related Articles Development and characterization of a triple combination Gene Therapy vector inhibiting HIV-1 multiplication. J Gene Med. 2008 Jul 21; Authors: Asparuhova MB, Barde I, Trono D, Schranz K, Schümperli D BACKGROUND: RNA-based approaches are promising for long-term Gene Therapy against HIV-1. They can target virtually any step of the viral replication cycle. It is also possible to combine anti-HIV-1 transgenes targeting different facets of HIV replication to compensate for limitations of any individual construct, maximizing efficacy and decreasing chances of escape mutations. We have previously developed two strategies to inhibit HIV-1 multiplication. One was a short hairpin RNA targeting the host factor cyclophilin A implicated in HIV-1 replication. Additionally, an antisense derivative of U7 small nuclear RNA was designed to induce the skipping of the HIV-1 Tat and Rev internal exons. RESULTS: In the present study, we have established an additional tRNAval promoter-driven shRNA against the coding sequence of viral infectivity factor. When human T-cell lines or primary CD4+ T cells are transduced with a triple lentiviral vector encoding these three therapeutic RNAs, HIV-1 multiplication is very efficiently suppressed. Moreover, all three therapeutic RNAs exhibit antiviral effects at early stages of the viral replication cycle (i.e. prior to viral cDNA integration or Gene expression). CONCLUSIONS: These findings make this ... Source: www.ncbi.nlm.nih.gov --- 1 day ago
Evaluation of E1A double mutant oncolytic adenovectors in anti-glioma Gene Therapy. J Med Virol. 2008 Jul 22;80(9):1595-1603 Authors: Ulasov IV, Tyler MA, Rivera AA, Nettlebeck DM, Douglas JT, Lesniak MS Malignant glioma, in particular glioblastoma multiforme (GBM), represents one of the most devastating cancers currently known and existing treatment regimens do little to change patient prognosis. Conditionally replicating adenoviral vectors (CRAds) represent attractive experimental anti-cancer agents with potential for clinical application. However, early protein products of the wild type adenovirus backbone-such as E1A-limit CRAds' replicative specificity. In this study, we evaluated the oncolytic potency and specificity of CRAds in which p300/CPB and/or pRb binding capacities of E1A were ablated to reduce non-specific replicative cytolysis. In vitro cytopathic assays, quantitative PCR analysis, Western blot, and flow cytometry studies demonstrate the superior anti-glioma efficacy of a double-mutated CRAd, Ad2/24CMV, which harbors mutations that reduce E1A binding to p300/CPB and pRb. When compared to its single-mutated and wild type counterparts, Ad2/24CMV demonstrated attenuated replication and cytotoxicity in representative normal human brain while displaying enhanced replicative cytotoxicity in malignant glioma. These results have implications for the development of double-mutated CRAd vectors for enhanced GBM Therapy. J. Med. Vi ... Source: www.ncbi.nlm.nih.gov --- 9 days ago
Related Articles Biologic Response of Human Intervertebral Disc Cells to Gene Therapy Cocktail. Spine. 2008 Jul 10; Authors: Moon SH, Nishida K, Gilbertson LG, Lee HM, Kim H, Hall RA, Robbins PD, Kang JD STUDY DESIGN.: In vitro experiment using human intervertebral disc (IVD) cells and adenovirus-therapeutic Gene constructs. OBJECTIVE.: To examine the biologic effect of "cocktail" therapeutic Gene transfer to human IVD cells in three-dimensional cultures. SUMMARY OF BACKGROUND DATA.: Gene Therapy is regarded as a potential option for the treatment of degenerative disc disease. Although various anabolic genes have previously been introduced for this purpose, cocktail Gene transfer of anabolic genes to IVD cells has never been attempted. METHODS.: Human IVDs were harvested during surgical disc procedures and cultured. We prepared recombinant adenovirus constructs bearing the TGF-beta1 Gene (Ad/TGF-beta1), the IGF-1 Gene (Ad/IGF-1), and the BMP-2 Gene (Ad/BMP-2). Transgene expression was detected by luciferase assays, enzyme linked immunosorbent assays, and Western blot analysis. Newly synthesized proteoglycan was measured by S-sulfate incorporation on Sephadex G-25 M in PD 10 columns. Human IVD cells were transduced by single, double, and triple combination of Ad/TGF-beta1, Ad/IGF-1, Ad/BMP-2 with an MOI of 75, then cultured three-dimensionally in alginate beads. RESULTS.: Transgene expression was detected at 18 hours after viral transd ... Source: www.moreover.com --- 7 days ago
Extract not available. ... Source: www.moreover.com --- 12 days ago
According to recent research published in the journal Proceedings of the National Academy of Sciences of the United States of America, 'The lysogenic state of bacteriophage lambda is maintained by CI repressor, which negatively regulates two promoters ... Source: www.ncbi.nlm.nih.gov --- 12 days ago
Related Articles Development of adenovirus immobilization strategies for in situ Gene Therapy. J Gene Med. 2008 Jul 10; Authors: Hu WW, Lang MW, Krebsbach PH BACKGROUND: Regenerative Gene Therapy using viral vectors enables transduced cells to express bioactive factors in vivo. Viral delivery with spatial control can enhance transduction efficiency and may limit systemic infection. Consequently, we tethered biotinylated adenovirus via interactions with avidin on chitosan surfaces to gain robust control for in situ transduction. METHODS: Avidin was either directly conjugated to chitosan (virus-biotin-avidin-material; VBAM) or indirectly docked on biotinylated chitosan surfaces (virus-biotin-avidin-biotin-material; VBABM) to tether biotinylated adenovirus. Enzyme-linked immunosorbent assay (ELISA) and spectroscopic analysis were performed to demonstrate the binding profiles. Biotin-alkaline phosphatase and biotinylated adenovirus were used as different sized particles to evaluate binding efficiencies and were compared by the Sips isotherm adsorption method. Scanning electron microscopy (SEM) examination illustrated virus distribution, and the transduction efficiency was determined by in vitro cell transduction. RESULTS: ELISA and spectroscopic analysis both demonstrated that the VBAM system led to multilayer avidin formation on biomaterial surfaces, whereas VBABM formed a monolayer of avidin. Sips isotherm adsorption indicated that the ... Source: www.moreover.com --- 5 days ago
Gene Therapy Scientists paint viruses to track their fate in the body Biologists from Austria and Singapore from the Christian Doppler Laboratory for Gene Therapeutic Vectors developed a technique that adds a new twist on the relationship between ... Gene Therapy - New Gene Therapy study findings have been reported by scientists at Aarhus University
Source: www.moreover.com --- 12 days ago
According to recent research from Aarhus, Denmark, 'Hyaluronan (HA) plays a predominant role in tissue morphogenesis, cell migration, proliferation, and . The aims of the present study were to investigate whether (i) prolonged presence of high ... Source: www.moreover.com --- 5 days ago
'rAAV (recombinant adeno-associated virus) has become a very useful Gene-delivery for . However, it is very difficult to generate rAAV using triple on a commercial scale, owing to its low transfection efficiency,' scientists in Shanghai, report. ... Source: www.moreover.com --- 12 days ago
According to recent research published in the Journal of Gene Medicine, ' We previously reported the 2-week benefits of platelet-derived growth factor (PD-ECGF) in chronically ischernic myocardium. ... Source: www.moreover.com --- 12 days ago
'The zinc-finger Yin Yang 1 (YY1) is a multifunctional that plays a critical role in development. ... Source: www.ncbi.nlm.nih.gov --- 24 days ago
Related Articles The possible curative Therapy for rheumatoid arthritis-EBV infection control Gene SAP and its application. Nihon Rinsho Meneki Gakkai Kaishi. 2008 Jun;31(3):141-51 Authors: Takei M, Kitamura N, Shiraiwa H, Inomata H, Nozaki T, Kuwana Y, Shiozaki M, Sawada S, Ishiwata T Epstein-Barr virus (EBV) belong to herpes virus group. This virus is transmitted by human contact and cause primary infection and may exist even for years in a latent state in healthy individuals. This virus may be reactivated by the dysregulation of the host immune system or possibly by virus mutation. Here we have firstly demonstrated the host defense of EBV infection and association of EBV with rheumatoid synovitis, and then discussed our own ideas of the possible treatment in near future. The key points of this new Therapy are SAP (signaling lymphocytic-activation molecule associated protein) or SH2D1A (Src homology 2 domain-containing protein). SAP (or SH2D1A), an adaptor-like molecule expressed in immune cells, is composed almost exclusively of a Src homology 2 (SH2) domain. In humans, SAP is mutated and either absent or non-functional in X-linked lymphoproliferative (XLP) syndrome (Duncan disease), a disease characterized by an inappropriate response to EBV infection. SAP is essential for late B cell help and the development of long-term humoral immunity. New approach to the therapeutic method for EBV might be opened by the regulation of this mole ... Source: www.moreover.com --- 12 days ago
'The T cell 3 (Tlx3) has been implicated in specification of glutamatergic sensory neurons in the . In cranial sensory ganglia, Tlx3 is highly expressed in differentiating neurons during early embryogenesis,' scientists writing in the journal ... Source: www.moreover.com --- 12 days ago
'In this study we have developed a useful method to identify a particular yeast strain within a mixture of strains during must , based on the presence or absence of a stable element derived from Saccharomyces cerevisiae 23S autonomous replicon. ... Source: www.moreover.com --- 12 days ago
'Nonsteroidal drugs ( NSAIDs) are used extensively as agents, despite their well documented ( GI) . ... Source: www.moreover.com --- 5 days ago
Talk about a good week for PTC. ... Source: www.moreover.com --- 12 days ago
According to recent research from Tsu, Japan, 'In adoptive transfer as an intervention for diseases, retroviral transfer of T-cell receptor ( TCR) genes derived from CD8(+) ( CTL) clones provides an opportunity to generate a large number of T cells ... Source: www.moreover.com --- 12 days ago
'The ferric uptake regulatory , Fur, functions as a global regulatory protein of in the mucosal Neisseria gonorrhoeae. ... Source: www.moreover.com --- 12 days ago
'A central issue in mediating repression by nuclear receptors is the distinct or redundant function between co-repressors N-CoR (nuclear receptor co-repressor) and SMRT (silencing mediator of and ... Find more results for Gene Therapy on RSSMicro.com |
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AFP - French researchers on Tuesday said that Gene Therapy, tested on lab animals, showed promise in combatting the tragic neurogenerative disease known as Huntington's. ...