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Source: www.rhapsody.com --- 6 days ago
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Source: www.ncbi.nlm.nih.gov --- 3 days ago
Related Articles Superior Osteogenic Capacity for Bone Tissue Engineering of Fetal Compared To Perinatal and Adult Mesenchymal Stem Cells. Stem Cells. 2008 Oct 2; Authors: Zhang ZY, Teoh SH, Chong MS, Schantz JT, Fisk NM, Choolani MA, Chan J Mesenchymal stem cells (MSC) from human Adult bone marrow (haMSC) represent a promising source for bone tissue engineering. However, their low frequencies and limited proliferation restrict clinical utility. Alternative post-natal, perinatal and fetal sources of MSC appear to have different osteogenic capacities, but have not been systematically compared with haMSC. We investigated the proliferative and osteogenic potential of MSC from human fetal bone marrow (hfMSC), human umbilical cord (hUCMSC), human Adult adipose tissue (hATMSC) and haMSC, both in monolayer cultures and after loading into 3-dimensional polycaprolactone-tricalcium-phosphate scaffolds. While all MSC had comparable immunophenotype, only hfMSC and hUCMSC were positive for the embryonic pluripotency markers Oct-4 and Nanog. hfMSC expressed the lowest HLA-I (55% vs 95-99%), the highest Stro-1 levels (51% vs 10-27%) and had the greatest CFU-F capacity (1.6-2.0x, p<0.01) and fastest doubling time (32 vs 54-111 hrs, p<0.01). hfMSC had the greatest osteogenic capacity, as assessed by von-Kossa staining, ALP activity (5.1-12.4x, p<0.01), calcium deposition (1.6-2.7x in monolayer and 1.6-5.0x in scaffold culture, p<0.01), calcium visualiz ... Source: www.ncbi.nlm.nih.gov --- 4 days ago
Related Articles Placenta--an Alternative source of stem cells. Toxicol Appl Pharmacol. 2005 Sep 1;207(2 Suppl):544-9 Authors: Matikainen T, Laine J The two most promising practical applications of human stem cells are cellular replacement therapies in human disease and toxicological screening of candidate drug molecules. Both require a source of human stem cells that can be isolated, purified, expanded in number and differentiated into the cell type of choice in a controlled manner. Currently, uses of both embryonic and Adult stem cells are investigated. While embryonic stem cells are pluripotent and can differentiate into any specialised cell type, their use requires establishment of embryonic stem cell lines using the inner cell mass of an early pre-implantation embryo. As the blastocyst is destroyed during the process, ethical issues need to be carefully considered. The use of embryonic stem cells is also limited by the difficulties in growing large numbers of the cells without inducing spontaneous differentiation, and the problems in controlling directed differentiation of the cells. The use of Adult stem cells, typically derived from bone marrow, but also from other tissues, is ethically non-controversial but their differentiation potential is more limited than that of the embryonic stem cells. Since human cord blood, umbilical cord, placenta and amnion are normally discarded at birth, they provide an easily accessible alternati ... Source: www.ncbi.nlm.nih.gov --- 9 days ago
Related Articles Updates on stem cells and their applications in regenerative medicine. J Tissue Eng Regen Med. 2008 Jun;2(4):169-83 Authors: Bajada S, Mazakova I, Richardson JB, Ashammakhi N Stem cells have the capacity for self-renewal and capability of differentiation to various cell lineages. Thus, they represent an important building block for regenerative medicine and tissue engineering. These cells can be broadly classified into embryonic stem cells (ESCs) and non-embryonic or Adult stem cells. ESCs have great potential but their use is still limited by several ethical and scientific considerations. The use of bone marrow-, umbilical cord-, adipose tissue-, skin- and amniotic fluid-derived mesenchymal stem cells might be an adequate Alternative for translational practice. In particular, bone marrow-derived stem cells have been used successfully in the clinic for bone, cartilage, spinal cord, cardiac and bladder regeneration. Several preclinical experimental studies are under way for the application of stem cells in other conditions where current treatment options are inadequate. Stem cells can be used to improve healthcare by either augmenting the body's own regenerative potential or developing new therapies. This review is not meant to be exhaustive but gives a brief outlook on the past, present and the future of stem cell-based therapies in clinical practice. PMID: 18493906 [PubMed - indexed for MEDLINE] ... Source: www.ncbi.nlm.nih.gov --- 23 days ago
Related Articles Prevalence and correlates of selected Alternative and complementary medicine in Adult renal transplant patients. Clin Transplant. 2008 Sep 11; Authors: Hess S, De Geest S, Halter K, Dickenmann M, Denhaerynck K Background/Significance: The use of complementary and Alternative medicine (CAM) has increased considerably in the general population in recent decades. However, the prevalence of CAM use in renal transplant patients has not yet been assessed. Aim: To determine the prevalence and correlates of CAM use among renal transplant patients (RTX) who are more than one year post transplant. Methods: This secondary data analysis used data from Part 1 of the Supporting Medication Adherence in Renal Transplantation (SMART) study, which tested prevalence, determinants and consequences of non-adherence regarding immunosuppressive medication in kidney transplant (renal TX) patients. The study's convenience sample consisted of 356 patients recruited from two Swiss outpatient transplant clinics during their annual examination visits (mean age: 53+/-13; male: 58%). The use of CAM as well as the specific kind of CAM used was assessed through structured interviews. Assessed CAM use correlates were age, gender, education, depressive symptomatology, comorbidity and smoking status. Relationships between CAM use and candidate correlates were explored using multiple logistic regression. Results: The prevalence of CAM use in this sample o ... Source: www.ncbi.nlm.nih.gov --- 6 days ago
Related Articles [Dysmenorrhea in pediatric and adolescent gynaecology] Ginekol Pol. 2008 Jul;79(7):499-503 Authors: Drosdzol A, Skrzypulec V Dysmenorrhea is the most common problem in pediatric and adolescent gynaecology and it reaches approximately 20-90% of adolescents and young Adult females. Dysmenorrhea in adolescent girls is usually primary and is associated with normal ovulatory cycles and with no pelvic pathology. Secondary dysmenorrhea, associated with some pelvic pathology, constitutes approximately 10% of the cases and its most frequent reasons are: endometriosis, pelvic inflammatory disease, congenital mullerian anomalies and ovarian cysts. Prostaglandins and leukotriens play a significant role in etiopathogenesis of the primary dysmenorrhea. The therapy of the primary dysmenorrheal in adolescent girls involves: nonsteroidal anti-inflammatory drugs for at least 3 months, combined with oral contraceptives for at least 3-6 menstrual cycles, as well as dietary supplementation, other Alternative therapies (vitamins, herbal remedies, acupuncture, TENS) and surgical treatment Secondary causes of dysmenorrhea should be considered in adolescents with dysmenorrhea who do not respond to the treatment. The role of the pediatric and adolescent gynaecologist is to diagnose the reason of symptoms, educate the patient, review effective treatment options as well as to restore normal daily functioning. PMID: 18819458 [PubMed - in process] ... Source: www.ncbi.nlm.nih.gov --- 29 days ago
Related Articles [The criteria of high priority, adequacy of research in the animal and Adult human models, and the absence of an Alternative according to section sign 5 of the German Stem Cell Act: the ethical perspective.] Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2008 Sep 7; Authors: Beckmann JP The German Stem Cell Act forbids, in principle, the importation und use of human embryonic stem cells, but provides for exceptions under specific conditions. It is the first Federal German law that grants an ethical review authority over the approval process. The legislature has indeed established the criteria for the work of the members of the "Central Ethics Commission for Stem Cell Research" appointed by the Federal Government. The three criteria include the following: 'high priority of the research goals', sufficiencyy of research in the animal and Adult human models' and 'the absence of an Alternative'. Together they legitimate the importation and use of human embryonic stem cells. This paper seeks to elucidate these three criteria, to analyze their dual function as ethical and as scientific concepts, and to illustrate the ways in which they are being used. PMID: 18773177 [PubMed - as supplied by publisher] ... Source: www.rhapsody.com --- 15 days ago
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HealthNews New Stem Cell Technique, a Safer Alternative to Embryonic Stem Cells eFluxMedia - 2 hours ago By Alice Carver Scientists have found a new and safer way to generate induced pluripotent stem cells (iPS), which are cells that have all the qualities of embryonic stem cells, but they are produced from Adult cells, an Alternative to the technique ... Researchers Report Stem Cell Advance Washington Post Stem cell bank launched at UMass Worcester Telegram Wall Street Journal - Reuters - Catholic News Agency - Boston Globe all 109 news articles ...