Related Articles The Stem Cell Marker CD133 (Prominin-1) is Expressed in Various Human Glandular Epithelia. J Histochem Cytochem. 2008 Jul 21; Authors: Karbanová J, Missol-Kolka E, Fonseca AV, Lorra C, Janich P, Hollerová H, Jászai J, Ehrmann J, Kolar Z, Liebers C, Arl S, Subrtova D, Freund D, Mokry J, Huttner WB, Corbeil D Human prominin-1 (CD133) is expressed by various stem and progenitor cells originating from diverse sources. In addition to stem cells its mouse orthologue is expressed in a broad range of adult epithelial cells, where it is selectively concentrated in their apical domain. The lack of detection of prominin-1 in adult human epithelia might be explained, at least in part, to the specificity of the widely used AC133 antibody, which recognizes an epitope that appears dependent on glycosylation. Here we decided to re-examine its expression in adult human tissues particularly in glandular epithelia using a novel monoclonal antibody (80B258) generated against the human prominin-1 polypeptide. In examined tissues, we observed the 80B258 immunoreactivity at the apical or apicolateral membranes of polarized cells. For instance, we found an expression in secretory serous and mucous cells as well as intercalated ducts of the large salivary and lacrimal glands. In sweat glands including the gland of Moll, 80B258 immunoreactivity was found in the secretory (eccrine and apocrine glands) and duct (eccrine glands) portion. In liver ...
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